Frozen Sections
Frozen Sections

Frozen Tissues

Frozen Section and Tissue Banking

Frozen Tissues include PrestoCHILL (The science of cryoembedding for high-quality frozen sections), PRESTO (Processor/stainer for high-quality frozen section, 6 stained slides in 5 minutes) and FlashFREEZE (The science of flash freezing for standardized biobanking)

  • Paper cryoembedding for small fragments.Milestone Medical
  • Innovative benchtop processor/stainer for high quality frozen section.
  • Set and modify protocols according to your specific requirements.
  • Assure high quality, consistent staining results independent from operator skill.
  • Slides are stained in 4 minutes and ready for coverslipping and examination.
  • Results obtained with porcine tissues in less than 10 minutes.

PrestoCHILL

The science of cryoembedding for high-quality frozen sections.

  • PrestoCHILLNo freezing artifacts.
  • 60 seconds freezing time.
  • An all-dry system.
  • Full downloadable documentation.
  • NO liquid nitrogen.
  • NO Co2.
  • NO isopentane.

The optimal frozen section begins with an evenly and completely frozen tissue block in which the block face to be sectioned is fl at and includes a complete, full faced section of the tissue submitted.
The section should be free of ice crystal artifacts, knife lines, or tears.
The speed and simplicity of operations of the PrestoCHILL revolutionize the rapid freezing process while keeping the “true to life” microscopic appearance of tissues.

Time to redefine frozen sections

  • Reduce freezing time to 60 seconds.
  • Eliminate freezing artifacts through ultrafast freezing, preventing formation of ice crystals.
  • Cut fatty tissues like breast, lymph nodes without diffi culty and without distortion of delicate honeycomb structure.
  • Obtain perfectly “fl at plane” surfaces for reduced trimming time and for easy cutting while using the patented* “face down” embedding technique.
  • Eliminate compression artifacts caused by ultra low temperatures.
  • Eliminate retraction of tissues: for example, Glomeruli from Bowman’s capsule, and vacuolation spaces around nucleated brain cells, compared to formaline fi xed, paraffi n embedded (FFPE) sections.

Cryoembedding of delicate tissues - small fragments/Mohs surgery

In the practice of frozen section pathology, pathologists are often faced with the task of embedding tissues that are extremely delicate because of the nature of the consistence and structure of the tissue.
This situation is a daily occurrence in Mohs surgery, where the technologist is faced with delicate slivers of skin with the goal of embedding this tissue precisely and completely visualizing the epidermis and margin.
The PrestoCHILL paper embedding technique offers a safe and rapid solution to these problems.

Fully automatic defrost feature

To eliminate potential formation of ice on the freezing platform, an automatic defrost cycle is provided. It consists of a heater embedded in the freezing platform and a vacuum pump to extract water vapors from the chamber. Vapors are condensed and collected in a cold-trap placed in the front of the unit for easy handling. A HEPA filter is provided on the exhaust side of the pump. Integrated software can set the defrost cycle to take place during off hours, then automatically, restart the cooling cycle at a preset time to ensure availability for the first case of the day. Removing the need to manually monitor the system.

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PRESTO

Processor/Stainer for high-quality frozen sections.

  • PrestoFully automated.
  • Fast: 4 minutes for 6 slides.
  • Quality results independent of operator skill.
  • Standardized staining results.
  • Open staining reagent platform.
  • Fully downloadable documentation.

The clinical laboratory relies heavily on the frozen section technique to provide intraoperative consultations when the surgeon requires a rapid diagnosis or immediate feedback relating to surgical margins.

Frozen sections provide a short turnaround time (15-20 minutes) but with the disadvantage of lower diagnostic quality than paraffi n sections due to cryoembedding artifacts and variability of staining protocols. A major reason for the lower quality versus a FFPE section is that frozen sections are only stained while FFPE sections are first processed (fixation + dehydration + clearing) and subsequently stained.

PRESTO is an innovative benchtop processor/stainer for high quality frozen sections. It features a standardized approach to fi rst processing the slide, with FineFIX (a Milestone proprietary solution) followed by fully automated staining protocols.

Improve morphology and total staining turnaround time

After 4 minutes slides are stained. Slides are now ready for coverslipping and examination.

PRESTO unique features

  • Improved morphology
  • High productivity
  • Fast
  • High flexibility
  • Full documentation
  • Safe

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FlashFREEZE

The science of flash freezing for standardized biobanking.

  • FlashFREEZEHigh flexibility.
  • Fast: only 1 hour 40 minutes to reach operating temperature of -80°C.
  • Safe: no liquid nitrogen, no isopentane.
  • Environmental friendly.
  • Full documentation.
  • Space saving.

The problem
There are few resources as valuable to cancer researchers as tissue samples - the biospecimens that patients donate to aid in the search for cancer-related biomarkers. Harvesting and storing human tissue for research purposes is not new.
Human biospecimens have been collected and stored for 100 years, and today, in excess of 300 million biospecimens are warehoused in freezers or stored in other formats such as paraffin-embedded tissue blocks. However, over the past decade or so it has become clear that the great majority of biospecimens stored in the world’s biorepositories may not be suited for the state-of-the-art genomics, proteomics, metabolomics, and other bioanalytical technologies used today to search for cancerrelated biomarkers. The irreproducibility of many reported biomarkers is due at least in part to the fact that the biospecimens utilized are often procured using different collection, processing and storage techniques. Such variability can lead to significant differences in biospecimen molecular integrity.

The solution
To overcome some of these difficulties Milestone has developed the FlashFREEZE unit to standardize the flash freezing processing step of biospecimen. With FlashFREEZE it is now possible to run evidence-based biospecimens freezing protocols with the full documentation needed for QA purposes.
FlashFREEZE uses the advantages of a state-of-the-art Stirling cooler, capable of reaching temperature down to -80°C rapidly.

FlashFREEZE has been designed, tested and manufactured to be operated without the use of isopentane or similar extremely flammable fluids.

Defrost feature
Defrost featureTo eliminate potential formation of ice on the freezing platform, a manual defrost cycle is provided. It consists of a heater embedded in the freezing platform and a vacuum pump to extract water vapors from the chamber. Vapors are condensed and collected in a cold-trap placed in the front of the unit for easy handling. A HEPA filter is provided on the exhaust side of the pump. Integrated software can set the defrost cycle to take place during off hours and restart the cooling cycle at a preset time to ensure availability for the first case of the day.

Read more...